Antibodies with infinite affinity

Antibodies with infinite affinity.

Here we report an approach to the design and production of antibody/ligand pairs, to achieve functional affinity far greater than avidin/biotin. Using fundamental chemical principles, we have developed antibody/ligand pairs that retain the binding specificity of the antibody, but do not dissociate. Choosing a structurally characterized antibody/ligand pair as an example, we engineered complemen...

Mepanipyrim haptens and antibodies with nanomolar affinity.

Mepanipyrim is an anilinopyrimidine fungicide used worldwide for crop protection. With the aim of developing useful immunoreagents for mepanipyrim immunoanalysis, two new functionalized derivatives were prepared and antibodies were generated. Affinity and specificity were assessed by direct and indirect competitive ELISA using homologous and heterologous conjugates. Although all antibodies were...

Binding Affinity of Antibodies?

Although the switch process is frequently associated with affinity maturation, the constant region is not assumed to play a role in Ag–Ab binding. In the present work, we demonstrate that two clonally related human monoclonal Igs sharing identical V H and V L sequences, but expressing different isotypes (IgA1 k PER and IgG1 k PER ), bind tubulin with significantly different affinities. This dif...

Antibodies Correlates Inversely with Affinity The Potency of Erythropoietin-Mimic

Presence of matrix-specific antibodies in affinity-purified polyclonal antibodies.

In general, antigen affinity columns made with commercially prepared activated affinity supports bind antibody specific for the coupled antigen. Nonetheless, in some cases affinity purification may yield antibodies to molecules other than the molecule of interest. In this report, we demonstrate such an occurrence: an antibody which adsorbs to an Affi-Prep 10 affinity matrix was found in the ser...